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monoclonal mouse anti lat1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology monoclonal mouse anti lat1
    Monoclonal Mouse Anti Lat1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+lat1+antibody/LAT1+Antibody/pm41317256-92-29-33
    Average 93 stars, based on 100 article reviews
    monoclonal mouse anti lat1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Enhancing the accumulation level of 3-[ 18 F]fluoro-L-α-methyltyrosine in tumors by preloading probenecid.
    Article Snippet: .. Then, blots were incubated with mouse anti-LAT1 antibody (sc374232, Santa Cruz Biotechnology, Dallas, TX) or rabbit anti-β-actin antibody (#4970, Cell Signaling Technology, Danvers, MA) at 4 °C overnight. .. After washing with TBST, the blots were incubated with horseradish peroxidase conjugated anti-rabbit or anti-mouse IgG antibody at room temperature for 1.5 h. The blots were further washed with TBST, and specific proteins were visualized using Clarity Western ECL Substrate (BioRad).

    Article Title: Enhancing the Therapeutic Effect of 2- 211 At-astato-α-methyl-L-phenylalanine with Probenecid Loading
    Article Snippet: .. Then the blots were incubated with mouse anti-LAT1 antibody (sc-374232, Santa Cruz Biotechnology, Dallas, TX, USA) or rabbit anti-β-actin antibody (#4970, Cell Signaling Technology, Danvers, MA, USA) at 4 °C overnight. .. After washing with TBST, the blots were incubated with horseradish peroxidase conjugated anti-rabbit or anti-mouse IgG antibodies at room temperature for 1.5 h. The blots were further washed with TBST, and specific proteins were visualized using Clarity Western ECL Substrate (BioRad).



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    Expression patterns of <t>LAT1,</t> LAT2, and 4 F2 hc in breast cancer subtypes. A RNA-seq data from TCGA, METABRIC, and HNCH-BC databases showing the expression levels of LAT1 ( SLC7 A5 ), LAT2 ( SLC7 A8 ), and 4 F2 hc ( SLC3 A2 ) across different breast cancer subtypes. Donut charts indicate the distribution of molecular subtypes in each database, while mountain plots depict the differences in transcript levels (*** p < 0.001). Immunohistochemistry (IHC) demonstrate LAT1 and LAT2 protein levels in tumor tissue samples. B Representative IHC images for LAT1 and LAT2 proteins in breast cancer subtypes ( n = 20/subtype), quantified by average optical density (AOD) (* p < 0.05; ** p < 0.01). C Distribution of LAT1 and LAT2 protein levels across breast cancer subtypes
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    Expression patterns of <t>LAT1,</t> LAT2, and 4 F2 hc in breast cancer subtypes. A RNA-seq data from TCGA, METABRIC, and HNCH-BC databases showing the expression levels of LAT1 ( SLC7 A5 ), LAT2 ( SLC7 A8 ), and 4 F2 hc ( SLC3 A2 ) across different breast cancer subtypes. Donut charts indicate the distribution of molecular subtypes in each database, while mountain plots depict the differences in transcript levels (*** p < 0.001). Immunohistochemistry (IHC) demonstrate LAT1 and LAT2 protein levels in tumor tissue samples. B Representative IHC images for LAT1 and LAT2 proteins in breast cancer subtypes ( n = 20/subtype), quantified by average optical density (AOD) (* p < 0.05; ** p < 0.01). C Distribution of LAT1 and LAT2 protein levels across breast cancer subtypes
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    Image Search Results


    Expression patterns of LAT1, LAT2, and 4 F2 hc in breast cancer subtypes. A RNA-seq data from TCGA, METABRIC, and HNCH-BC databases showing the expression levels of LAT1 ( SLC7 A5 ), LAT2 ( SLC7 A8 ), and 4 F2 hc ( SLC3 A2 ) across different breast cancer subtypes. Donut charts indicate the distribution of molecular subtypes in each database, while mountain plots depict the differences in transcript levels (*** p < 0.001). Immunohistochemistry (IHC) demonstrate LAT1 and LAT2 protein levels in tumor tissue samples. B Representative IHC images for LAT1 and LAT2 proteins in breast cancer subtypes ( n = 20/subtype), quantified by average optical density (AOD) (* p < 0.05; ** p < 0.01). C Distribution of LAT1 and LAT2 protein levels across breast cancer subtypes

    Journal: Amino Acids

    Article Title: Targeting LAT1 with JPH203 to reduce TNBC proliferation and reshape suppressive immune microenvironment by blocking essential amino acid uptake

    doi: 10.1007/s00726-025-03456-3

    Figure Lengend Snippet: Expression patterns of LAT1, LAT2, and 4 F2 hc in breast cancer subtypes. A RNA-seq data from TCGA, METABRIC, and HNCH-BC databases showing the expression levels of LAT1 ( SLC7 A5 ), LAT2 ( SLC7 A8 ), and 4 F2 hc ( SLC3 A2 ) across different breast cancer subtypes. Donut charts indicate the distribution of molecular subtypes in each database, while mountain plots depict the differences in transcript levels (*** p < 0.001). Immunohistochemistry (IHC) demonstrate LAT1 and LAT2 protein levels in tumor tissue samples. B Representative IHC images for LAT1 and LAT2 proteins in breast cancer subtypes ( n = 20/subtype), quantified by average optical density (AOD) (* p < 0.05; ** p < 0.01). C Distribution of LAT1 and LAT2 protein levels across breast cancer subtypes

    Article Snippet: The antibodies used included PerCP-conjugated mouse monoclonal anti-LAT1 antibody (#NBP2-50465PCP, Novus Biologicals), unconjugated mouse monoclonal anti-LAT2 antibody (#MA5-24,943, Invitrogen), and Alexa Fluor 488 goat anti-mouse secondary antibody (#A28175, Invitrogen).

    Techniques: Expressing, RNA Sequencing, Immunohistochemistry

    Therapeutic potential of targeting LAT1 in TNBC. A Quantitative analysis of LAT1 and LAT2 transcript levels in five breast cancer cell lines (MDA-MB-231, HCC1937, SK-BR-3, BT474, and T47D) using qRT-PCR, normalized to GAPDH and plotted as fold changes relative to T47D. B Flow cytometry analysis showing LAT1 and LAT2 protein expression on the cell surface of breast cancer cell lines, expressed as mean fluorescence intensity (MFI). C LAT1 knockdown using shRNA (shSLC7 A5-1 or shSLC7 A5-2) in MDA-MB-231 and HCC1937 cells. Flow cytometry validated LAT1 expression levels (Bank: wild type; shCtrl: empty shRNA). Proliferation was assessed using the CCK-8 assay (*** p < 0.001), and EAA levels were analyzed via metabolomics (*** p < 0.001). Data are presented as mean ± SD from three independent experiments

    Journal: Amino Acids

    Article Title: Targeting LAT1 with JPH203 to reduce TNBC proliferation and reshape suppressive immune microenvironment by blocking essential amino acid uptake

    doi: 10.1007/s00726-025-03456-3

    Figure Lengend Snippet: Therapeutic potential of targeting LAT1 in TNBC. A Quantitative analysis of LAT1 and LAT2 transcript levels in five breast cancer cell lines (MDA-MB-231, HCC1937, SK-BR-3, BT474, and T47D) using qRT-PCR, normalized to GAPDH and plotted as fold changes relative to T47D. B Flow cytometry analysis showing LAT1 and LAT2 protein expression on the cell surface of breast cancer cell lines, expressed as mean fluorescence intensity (MFI). C LAT1 knockdown using shRNA (shSLC7 A5-1 or shSLC7 A5-2) in MDA-MB-231 and HCC1937 cells. Flow cytometry validated LAT1 expression levels (Bank: wild type; shCtrl: empty shRNA). Proliferation was assessed using the CCK-8 assay (*** p < 0.001), and EAA levels were analyzed via metabolomics (*** p < 0.001). Data are presented as mean ± SD from three independent experiments

    Article Snippet: The antibodies used included PerCP-conjugated mouse monoclonal anti-LAT1 antibody (#NBP2-50465PCP, Novus Biologicals), unconjugated mouse monoclonal anti-LAT2 antibody (#MA5-24,943, Invitrogen), and Alexa Fluor 488 goat anti-mouse secondary antibody (#A28175, Invitrogen).

    Techniques: Quantitative RT-PCR, Flow Cytometry, Expressing, Fluorescence, Knockdown, shRNA, CCK-8 Assay